Estudio histoenzimático e inmunohistoquímico del cóndilo mandibular fetal humano.
- R. Berraquero Delgado
- J. Palacios Calvo
- B. Wucherpfenni Gaminde
- J.I. Rodríguez González
- C. Gamallo Amat
ISSN: 0210-1637
Year of publication: 1995
Volume: 36
Issue: 6
Pages: 318-326
Type: Article
More publications in: Ortodoncia española: Boletín de la Sociedad Española de Ortodoncia
Abstract
This paper analyses with histochemical methods, alcaline phosphatase (FA), adenosin triphosphatase (ATPase) and NADH-dehydrogenase (NADH-DH). Inmunohistochemical methods also allow us to study lysosomal cathepsine D protease presence, S-100 protein and lysozime (muramidase). The outcomes obtained show functional differences between the variety of celular types from the human foetal mandibular condyle. So, articular layer cells have NADH-DH and cathepsine D indicating its importance in synthesis and degradation of extracelular matrix components. Progenitor layer cells with an intensive NADH-DH enzyme activity have FA, probably related to its double potential capacity of converting themselves in chondrocytes and in bone cells. Intense hypertrophic positivity of chondrocytes towards FA, ATPase, S-100 protein and lisozyme as well as FA and ATPase activity in extracellular matrix of this layer are related to cartilage mineralization. Present observations are considered basic for future studies on changes of this proteins in posterior life stages, after biomechanic modifications or in pathological situations like temporomandibular osteoarthritis.