Induction of more aggressive tumoral phenotypes in LNCaP and PC3 cells by serum exosomes from prostate cancer patients.

  1. Recio Aldavero, Jorge 1
  2. Arenas Jiménez, María Isabel 2
  3. Román Curto, Irene Dolores 1
  4. Huertas Lárez, Raquel 1
  5. Muñoz Moreno, Laura 1
  6. Blasco Martínez, Ana 3
  7. Sanchís Bonet, Ángeles 4
  8. Bajo Chueca, Ana María 1
  1. 1 (Universidad de Alcalá. Departamento de Biología de Sistemas. Unidad de Bioquímica y Biología Molecular.)
  2. 2 (Universidad de Alcalá. Departamento de Biomedicina y Biotecnología. Unidad de Biología Celular)
  3. 3 (Hospital Príncipe de Asturias. Servicio de Anatomía Patológica)
  4. 4 (Hospital Príncipe de Asturias. Servicio de Urología)
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Éditeur: e-cienciaDatos

Année de publication: 2024

Type: Dataset

CC BY-NC-SA 4.0

DOI: 10.21950/JWF8EF lock_openAccès ouvert editor

Résumé

In recent years, the potential participation of exosomes in this process has been suggested in prostate cancer progression. We aim to study the effect of exosomes isolated from the serum of patients with PCa on various cellular processes associated with increased tumor aggressiveness in two PCa cell lines: LNCaP-FGC and PC3. The presence of exosomes was confirmed by immunodetection of specific markers and their size distribution was analyzed by Dynamic Light Scattering (DLS). The results obtained demonstrated that serum exosomes from PCa patients increased migration of PC3 cells and neuroendocrine differentiation of LNCaP-FGC cells regardless of the grade of the tumor. PCa serum exosomes also enhanced the secretion of enzymes related to invasiveness and resistance to chemotherapeutics, such as extracellular matrix metalloproteases 2 and 9, and gamma-glutamyltransferase in both cell lines. These findings support the pivotal participation of exosomes released by tumoral cells in the progression of PCa. Western blotting (WB); Zimography; Transmission electron microscopy (TEM)